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[Simultaneous Determination of Trillin and Desgalactotigonin Contents in Solanum Lyratum by HPLC-ELSD]

[Simultaneous Determination of Trillin and Desgalactotigonin Contents in Solanum Lyratum by HPLC-ELSD]

Yurong Jia, Changshan Sun, Yanxiu Sun, Wenwen Yang, Lixin Sun

Zhongguo Zhong Yao Za Zhi. 2012 Feb;37(3):323-6.

PMID: 22568233

Abstract:

Objective:
To establish a HPLC-ELSD method for simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum.
Method:
A Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) was adopted, with the mobile phase consisting of acetonitrile-10 mmol x L(-1) ammonium acetate (52: 48). The temperature was 25 degrees C, the flow rate was set at 0.6 mL x min(-1), and the sample size is 20 microL. The temperature of drift tubes and gas flow rate of the detector were set at 95 degrees C and 2.3 L x min(-1), respectively.
Result:
With in the linear ranges of 20-200 mg x L(-1) and 10-100 mg x L(-1), trillin and desgalactotigonin show a good linear relationship. The average recovery was 99.4% (RSD 0.90%) for trillin and 100.3% (RSD 1.1%) for desgalactotigonin.
Conclusion:
The method is so accurate and easily reproducible that it is suitable for the quality control of S. lyratum medicinal materials.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14144060	Trillin		14144-06-0	Price

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