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[Simultaneous Isolation and Purification of Gallic Acid and Brevifolincarboxylic Acid From Polygonum Capitatum by High-Speed Counter-Current Chromatography]

[Simultaneous Isolation and Purification of Gallic Acid and Brevifolincarboxylic Acid From Polygonum Capitatum by High-Speed Counter-Current Chromatography]

Xinxia Chen, Liyan Zhang, Jinzhi Wan, Bin Liang, Yu Xie

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(15):1957-60.

PMID: 20931845

Abstract:

Objective:
To isolate and purify gallic acid and brevifolincarboxylic acid simultaneously by high-speed counter-current chromatography (HSCCC) from a crude extract of Polygonum capitatum.
Method:
The biphasic solvent system composed of ethyl acetate-n-butanol-0.44% acetic acid (3:1:5) was used at a flow rate of 2.0 mL x min(-1), while the aqueous phase was selected as the mobile phase and the apparatus was rotated at 860 r x min(-1). The effluent was detected at 272 nm.
Result:
51.5 mg of gallic acid and 5.9 mg of brevifolincarboxylic acid were separated from 1.07 g of the crude extract with the purities of 99.7% and 97.5%, respectively, while brevifolincarboxylic acid was obtained firstly from the genus Polygonum. The structures of the compounds were identified by ultraviolet spectrometry (UV), infra-red spectrometry (IR), liquid chromatography/mass spectrometry (LC/MS), time-of-flight mass spectrometry( TOF-MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR.
Conclusion:
This method is feasible and rapid for isolation and purification of gallice acid and brevifolincarboxylil acid.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18490954	Brevifolincarboxylic acid		18490-95-4	Price

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