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Simultaneous Quantification of Alprazolam, 4- And Alpha-Hydroxyalprazolam in Plasma Samples Using Liquid Chromatography Mass Spectrometry

Simultaneous Quantification of Alprazolam, 4- And Alpha-Hydroxyalprazolam in Plasma Samples Using Liquid Chromatography Mass Spectrometry

Annika Allqvist, Agneta Wennerholm, Jan-Olof Svensson, Rajaa A Mirghani

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jan 5;814(1):127-31.

PMID: 15607716

Abstract:

A sensitive and specific method was developed for quantification of alprazolam and its two metabolites 4-hydroxyalprazolam and alpha-hydroxyalprazolam in plasma. The work up procedure was solid phase extraction. Liquid chromatography-mass spectrometry (LC-MS) was used for separation, detection and quantification of the analytes. The limit of quantitation (LOQ) was 0.05 ng/mL for alprazolam and the two metabolites. The extraction recovery was more than 82% for alprazolam and its metabolites. The within- and between-assay coefficients of variation were in the range of 1.9-17.9%. The method was used for determination of the pharmacokinetics parameters of alprazolam and its two metabolites in healthy Caucasian subjects who ingested 1mg of alprazolam.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP37115438-B	α-Hydroxyalprazolam		37115-43-8	Price

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