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Simultaneous Separation of Three Isomeric Sennosides From Senna Leaf (Cassia Acutifolia) Using Counter-Current Chromatography

Simultaneous Separation of Three Isomeric Sennosides From Senna Leaf (Cassia Acutifolia) Using Counter-Current Chromatography

Sait Byul Park, Yeong Shik Kim

J Sep Sci. 2015 Oct;38(20):3502-7.

PMID: 26255810

Abstract:

Senna leaf is widely consumed as tea to treat constipation or to aid in weight loss. Sennoside A, A1 , and B are dirheinanthrone glucosides that are abundant and the bioactive constituents in the plant. They are isomers that refer to the (R*R*), (S*S*), and (R*S*) forms of protons on C-10 and C-10' centers and it is difficult to refine them individually due to their structural similarities. The new separation method using counter-current chromatography successfully purified sennoside A, A1 , and B from senna leaf (Cassia acutifolia) while reversed-phase medium-pressure liquid chromatography yielded sennoside A only. n-Butanol/isopropanol/water (5:1:6, v/v/v) was selected as the solvent system for counter-current chromatography operation, and the partition coefficients were carefully determined by adding different concentrations of formic acid. High-resolution mass spectrometry and NMR spectroscopy were performed to verify the chemical properties of the compounds.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP66575302	Sennoside A1		66575-30-2	Price

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