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Simultaneous Ultrasound-Assisted Water Extraction and β-cyclodextrin Encapsulation of Polyphenols From Mangifera Indica Stem Bark in Counteracting TNFα-induced Endothelial Dysfunction

Simultaneous Ultrasound-Assisted Water Extraction and β-cyclodextrin Encapsulation of Polyphenols From Mangifera Indica Stem Bark in Counteracting TNFα-induced Endothelial Dysfunction

Marzia Mura, Daniela Palmieri, Davide Garella, Antonella Di Stilo, Patrizia Perego, Giancarlo Cravotto, Domenico Palombo

Nat Prod Res. 2015;29(17):1657-63.

PMID: 25589236

Abstract:

This study proposes an alternative technique to prevent heat degradation induced by classic procedures of bioactive compound extraction, comparing classical maceration/decoction in hot water of polyphenols from Mango (Mangifera indica L.) (MI) with ultrasound-assisted extraction (UAE) in a water solution of β-cyclodextrin (β-CD) at room temperature and testing their biological activity on TNFα-induced endothelial dysfunction. Both extracts counteracted TNFα effects on EAhy926 cells, down-modulating interleukin-6, interleukin-8, cyclooxygenase-2 and intracellular adhesion molecule-1, while increasing endothelial nitric oxide synthase levels. β-CD extract showed higher efficacy in improving endothelial function. These effects were abolished after pre-treatment with the oestrogen receptor inhibitor ICI1182,780. Moreover, the β-CD extract induced Akt activation and completely abolished the TNFα-induced p38MAPK phosphorylation. UAE and β-CD encapsulation provide an efficient extraction protocol that increases polyphenol bioavailability. Polyphenols from MI play a protective role on endothelial cells and may be further considered as oestrogen-like molecules with vascular protective properties.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415499	InSolution p38 MAP Kinase Inhibitor III			Price

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