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Single-molecule Spectroscopy Using Nanoporous Membranes

Single-molecule Spectroscopy Using Nanoporous Membranes

Guillaume A T Chansin, Rafael Mulero, Jongin Hong, Min Jun Kim, Andrew J DeMello, Joshua B Edel

Nano Lett. 2007 Sep;7(9):2901-6.

PMID: 17718589

Abstract:

We describe a novel approach for optically detecting DNA translocation events through an array of solid-state nanopores that potentially allows for ultra high-throughput, parallel detection at the single-molecule level. The approach functions by electrokinetically driving DNA strands through sub micrometer-sized holes on an aluminum/silicon nitride membrane. During the translocation process, the molecules are confined to the walls of the nanofluidic channels, allowing 100% detection efficiency. Importantly, the opaque aluminum layer acts as an optical barrier between the illuminated region and the analyte reservoir. In these conditions, high-contrast imaging of single-molecule events can be performed. To demonstrate the efficiency of the approach, a 10 pM fluorescently labeled lambda-DNA solution was used as a model system to detect simultaneous translocation events using electron multiplying CCD imaging. Single-pore translocation events are also successfully detected using single-point confocal spectroscopy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
CS3104387	Lambda DNA solution			Price

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