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Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters

Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters

Sunil Kumar Sailapu, Deepanjalee Dutta, Amaresh Kumar Sahoo, Siddhartha Sankar Ghosh, Arun Chattopadhyay

ACS Omega. 2018 Feb 28;3(2):2119-2129.

PMID: 30023824

Abstract:

A single platform for gene and protein expression studies is proposed to pursue rapid diagnostics. A common method to synthesize gold (Au) nanoclusters on both DNA and protein template was developed using a benchtop device. The method of synthesis is rapid and versatile and can be applied to different classes of DNA/protein. Employing luminescent Au nanoclusters as the signal-generating agents, the device enables carrying out reverse transcriptase polymerase chain reaction and array-based analyses of multiple genes/proteins simultaneously using switchable holders and custom-designed software. The device and methods were applied to evaluate gene profiling related to apoptosis in HeLa cancer cells and further to analyze the protein expressions of glutathione-S-transferase (GST) and GST-tagged human granulocyte macrophage colony-stimulating factor (GST-hGMCSF) recombinant proteins purified from bacterial strains of BL21(DE3) Escherichia coli (E. coli). The device with user-friendly methods for diagnosis using the luminescence of Au nanoclusters offers potential use in disease diagnostics with a vision to extend health care facilities especially to remote geographical locations.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42415012	P73b, GST tagged human			Price

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