Home
Resources
Publications
Site-specific Conjugation of a Temperature-Sensitive Polymer to a Genetically-Engineered Protein

Site-specific Conjugation of a Temperature-Sensitive Polymer to a Genetically-Engineered Protein

A Chilkoti, G Chen, P S Stayton, A S Hoffman

Bioconjug Chem. Nov-Dec 1994;5(6):504-7.

PMID: 7873655

Abstract:

A genetically-engineered mutant of cytochrome b5, incorporating a unique cysteine residue, was conjugated to maleimide-terminated oligo(N-isopropylacrylamide). The conjugation of the protein by reaction of the cysteine residue, precisely positioned by site-directed mutagenesis techniques, with an activated oligomer containing only one reactive end group in the oligomer chain permits the site-specific and stoichiometric conjugation of the oligomer with the protein. The protein-oligomer conjugate was shown to exhibit lower critical solution temperature (LCST) behavior, similar to the free oligomer. Furthermore, the LCST behavior of the protein-oligomer conjugate is reversible and allows selective precipitation of the conjugate above its LCST.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS74678	Poly(N-isopropylacrylamide), maleimide terminated			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: