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Skp-cullin-F Box E3 Ligase Component FBXL2 Ubiquitinates Aurora B to Inhibit Tumorigenesis

Skp-cullin-F Box E3 Ligase Component FBXL2 Ubiquitinates Aurora B to Inhibit Tumorigenesis

B B Chen, J R Glasser, T A Coon, R K Mallampalli

Cell Death Dis. 2013 Aug 8;4(8):e759.

PMID: 23928698

Abstract:

Aurora B kinase is an integral regulator of cytokinesis, as it stabilizes the intercellular canal within the midbody to ensure proper chromosomal segregation during cell division. Here we identified that the ubiquitin E3 ligase complex SCF(FBXL2) mediates Aurora B ubiquitination and degradation within the midbody, which is sufficient to induce mitotic arrest and apoptosis. Three molecular acceptor sites (K¹⁰², K¹⁰³ and K²⁰⁷) within Aurora B protein were identified as important sites for its ubiquitination. A triple Lys mutant of Aurora B (K¹⁰²/¹⁰³/(²⁰⁷R)) exhibited optimal resistance to SCF(FBXL2)-directed polyubiquitination, and overexpression of this variant resulted in a significant delay in anaphase onset, resulting in apoptosis. A unique small molecule F-box/LRR-repeat protein 2 (FBXL2) activator, BC-1258, stabilized and increased levels of FBXL2 protein that promoted Aurora B degradation, resulting in tetraploidy, mitotic arrest and apoptosis of tumorigenic cells, and profoundly inhibiting tumor formation in athymic nude mice. These findings uncover a new proteolytic mechanism targeting a key regulator of cell replication that may serve as a basis for chemotherapeutic intervention in neoplasia.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1507370402	BC-1258		1507370-40-2	Price

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