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Small Intestinal Permeability and Gut-Transit Time Determined With Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice

Small Intestinal Permeability and Gut-Transit Time Determined With Low and High Molecular Weight Fluorescein Isothiocyanate-Dextrans in C3H Mice

Anni Woting, Michael Blaut

Nutrients. 2018 May 28;10(6):685.

PMID: 29843428

Abstract:

Protocols for intestinal permeability measurements in mice using 4-kDa fluorescein isothiocyanate-conjugated (FITC) dextran differ considerably among laboratories on the blood-sampling time. To find the optimal point in time for blood sampling, we administered 4-kDa FITC dextran to C3H mice and monitored the marker in plasma over 8 h. We also determined gut-transit time using 70-kDa FITC dextran, which does not cross the intestinal epithelium. The 4-kDa FITC dextran concentration in plasma reached its maximum 45 min after administration. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS74849	Fluorescein isothiocyanate - Dextran 500000-Conjugate			Price

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