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Sodium Ascorbate Kills Candida Albicans in Vitro via Iron-Catalyzed Fenton Reaction: Importance of Oxygenation and Metabolism

Sodium Ascorbate Kills Candida Albicans in Vitro via Iron-Catalyzed Fenton Reaction: Importance of Oxygenation and Metabolism

Pinar Avci, Fernanda Freire, Andras Banvolgyi, Eleftherios Mylonakis, Norbert M Wikonkal, Michael R Hamblin

Future Microbiol. 2016 Dec;11(12):1535-1547.

PMID: 27855492

Abstract:

Aim:
Ascorbate can inhibit growth and even decrease viability of various microbial species including Candida albicans. However the optimum conditions and the mechanism of action are unclear. Materials/methodology: Candida albicans shaken for 90 min in a buffered solution of ascorbate (90 mM) gave a 5-log reduction of cell viability, while there was no killing without shaking, in growth media with different carbon sources or at 4°C. Killing was inhibited by the iron chelator 2,2'-bipyridyl. Hydroxyphenyl fluorescein probe showed the intracellular generation of hydroxyl radicals.
Results/conclusion:
Ascorbate-mediated killing of C. albicans depends on oxygenation and metabolism, involves iron-catalyzed generation of hydroxyl radicals via Fenton reaction and depletion of intracellular NADH. Ascorbate could serve as a component of a topical antifungal therapy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414867	Hydroxyphenyl fluorescein solution			Price

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