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Sodium-dependent Transport of L-leucine in Membrane Vesicles Prepared From Pseudomonas Aeruginosa

Sodium-dependent Transport of L-leucine in Membrane Vesicles Prepared From Pseudomonas Aeruginosa

T Hoshino, M Kageyama

J Bacteriol. 1979 Jan;137(1):73-81.

PMID: 83991

Abstract:

Membrane vesicles were prepared by osmotic lysis of spheroplasts of Pseudomonas aeruginosa strain P14, and the active transport of amino acids was studied. D-Glucose, gluconate, and L-malate supported active transport of various L-amino acids. The respiration-dependent leucine transport was markedly stimulated by Na+. Moreover, without any respiratory substrate, leucine was also transported transiently by the addition of Na+ alone. This transient uptake of leucine was not inhibited either by carbonyl cyanide p-trifluoromethyoxyphenylhydrazone or by valinomycin, but was completely abolished by gramicidin D. Increase in the concentration of Na+ of the medium resulted in a decrease of the Km for L-leucine transport, whereas the Vmax was not significnatly affected. Active transport of leucine was inhibited competitively by isoleucine or by valine, whose transport was also stimulated by Na+. On the other hand, Na+ was not required for the uptake of other L-amino acids tested, but rather was inhibitory for some of them. These results show (i) that a common transport system for branched-chain amino acids exists in membrane vesicles, (ii) that the system requires Na+ for its activity, and (iii) that an Na+ gradient can drive the system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP63474373	Sodium D,L-malate		63474-37-3	Price

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