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Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis of Polyethylene Glycolylated Interferon Alpha

Sodium Dodecyl Sulfate-Capillary Gel Electrophoresis of Polyethylene Glycolylated Interferon Alpha

Dong H Na, Eun J Park, Yu S Youn, Byung W Moon, Yeong W Jo, Sung H Lee, Won-Bae Kim, Yeowon Sohn, Kang C Lee

Electrophoresis. 2004 Feb;25(3):476-9.

PMID: 14760640

Abstract:

Sodium dodecyl sulfate-capillary gel electrophoresis (SDS-CGE) using a hydrophilic replaceable polymer network matrix was applied to characterize the polyethylene glycol(PEG)ylated interferon alpha (PEG-IFN). The SDS-CGE method resulted in a clearer resolution in both the PEG-IFN species and the native IFN species. The distribution profile of PEGylation determined by SDS-CGE was consistent with that obtained by SDS-polyacrylamide gel electrophoresis (PAGE) with Coomassie blue or barium iodide staining. The result was also compared using matrix-assisted laser desorption/ionization-time of flight-mass spectrometry. SDS-CGE was also useful for monitoring the PEGylation reaction to optimize the reaction conditions, such as reaction molar ratio. This study shows the potential of SDS-CGE as a new method for characterizing the PEGylated proteins with advantages of speed, minimal sample consumption and high resolution.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP13718508	Barium iodide		13718-50-8	Price

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