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Sodium Dodecyl Sulfate Cytotoxicity Towards HaCaT Keratinocytes: Comparative Analysis of Methods for Evaluation of Cell Viability

Sodium Dodecyl Sulfate Cytotoxicity Towards HaCaT Keratinocytes: Comparative Analysis of Methods for Evaluation of Cell Viability

A L Rusanov, N G Luzgina, A V Lisitsa

Bull Exp Biol Med. 2017 Jun;163(2):284-288.

PMID: 28726210

Abstract:

Viability of keratinocytes of HaCaT immortalized line incubated with sodium dodecyl sulfate for 3 min, 1 and 48 h, was studied by light microscopy, MTT test, and neutral red absorption test. The IC50 values were determined for each of the studied lengths of exposure. HaCaT cells exhibited a dose-dependent decrease of viability under the effect of sodium dodecyl sulfate, proportional to the length of exposure. The values measured by different methods (MTT test and neutral red absorption test) varied, the differences were determined by the duration of exposure to sodium dodecyl sulfate. The dispersion of values for 1 and 48 h exposure, obtained by MTT method, was greater than of the values obtained by neutral red absorption test.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP151213-B	Sodium dodecyl sulfate		151-21-3	Price

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