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Sodium Dodecyl Sulfate Free Gel electrophoresis/electroelution Sorting for Peptide Fractionation

Sodium Dodecyl Sulfate Free Gel electrophoresis/electroelution Sorting for Peptide Fractionation

Yassel Ramos, Annia González, Patricia Sosa-Acosta, Yasset Perez-Riverol, Yairet García, Lila Castellanos-Serra, Jeovanis Gil, Aniel Sánchez, Luis J González, Vladimir Besada

J Sep Sci. 2019 Dec;42(24):3712-3717.

PMID: 31625274

Abstract:

Shotgun proteomics based on peptide fractionation by using liquid chromatography has become the common procedure for proteomic studies, although in the very beginning of the field, protein separation by using electrophoresis was the main tool. Nonetheless, during the last two decades, the electrophoretic techniques for peptide mixtures fractionation have evolved as a result of relevant technological improvements. We also proposed the combination of sodium dodecyl sulfate polyacrylamide gel electrophoresis for protein fractionation and sodium dodecyl sulfate free polyacrylamide gel electrophoresis for peptide separation as a novel procedure for proteomic studies. Here, we present an optimized device for sodium dodecyl sulfate free polyacrylamide gel electrophoresis improving peptide recoveries respect to the established electrophoretic technique off gel electrophoresis meanwhile conserving the excellent resolution described for the former technique in slab gel based systems. The device simultaneously allows the separation and the collection of fractionated peptides in solution.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP151213-B	Sodium dodecyl sulfate		151-21-3	Price

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