Home
Resources
Publications
Solid-state 13C and 15N Nuclear Magnetic Resonance Studies of Alanine Metabolism in Aerococcus Viridans (Gaffkya Homari)

Solid-state 13C and 15N Nuclear Magnetic Resonance Studies of Alanine Metabolism in Aerococcus Viridans (Gaffkya Homari)

G S Jacob, J Schaefer, G E Wilson Jr

J Biol Chem. 1985 Mar 10;260(5):2777-81.

PMID: 3972803

Abstract:

Transport and metabolism of D- and L-alanine by Aerococcus viridans (Gaffkya homari) have been studied using cross-polarization magic-angle spinning 13C and 15N NMR spectroscopy of lyophilized whole cells, isolated cell walls, and crude extracts. For equimolar concentrations in the growth medium, about 10 times more D-alanine than L-alanine is transported into the cells. Examination of cells labeled with D-[13C]alanine and L-[epsilon-15N]lysine by double cross-polarization magic-angle spinning 15N NMR indicates that only about 20% of the D-alanine present in cell-wall peptidoglycan comes directly from the growth medium. The rest is produced by de novo synthesis. Most of the labeled D-alanine is found within peptidoglycan precursors or inverted to L-alanyl residues of soluble proteins.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411320	IsoYeast-13C,15N - Growth Medium			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: