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Spatial and Temporal Expression of CCR3 and the Common Beta Chain of the IL-3, IL-5 and GM-CSF Receptor in the Nasal Epithelium and Lymphoid Tissues in a Rat Model of Allergic Rhinitis

Spatial and Temporal Expression of CCR3 and the Common Beta Chain of the IL-3, IL-5 and GM-CSF Receptor in the Nasal Epithelium and Lymphoid Tissues in a Rat Model of Allergic Rhinitis

Marylène Fortin, James G Wagner, Julie Brault, Jack R Harkema, Paolo M Renzi, Luc Paquet, Nicolay Ferrari

Cytokine. 2010 Dec;52(3):194-202.

PMID: 20696593

Abstract:

Background:
Allergic rhinitis (AR) and asthma are closely related conditions that often co-exist, and are characterized by a Th2 inflammatory response where eosinophils occupy a predominant role. Strategies aimed at blocking signaling through the CC chemokine receptor 3 (CCR3) and/or the common beta chain of the IL-3, IL-5 and GM-CSF receptor (βc) efficiently reduced eosinophilic inflammation in both animal models and in asthmatic patients. This study was therefore aimed at characterizing the spatio-temporal expression pattern of βc and CCR3 using a rat model of AR.
Methods:
Sensitized rats were challenged with ovalbumin and sacrificed at 2h, 8h, 16h or 24h post-challenge. Nasal tissues were microdissected and used for mRNA quantification by QPCR, while histological evaluation determined the presence of eosinophils and mucosubstances.
Results:
Allergen-induced recruitment of eosinophils in the distal septum and turbinates was maximal at 8h post-challenge, and was correlated with 2-4-fold increase in CCR3 and βc mRNA. Recruitment of eosinophils was also accompanied by upregulated IL-5, IL-4Rα, TNF-α and IFN-γ mRNA at early time-points. In contrast, IL-13 and MUC5AC mRNA, as well as production of mucosubstances were maximal at 24h.
Conclusions:
βc and CCR3 could play important roles in the modulation of the allergic response, and their inhibition may represent a promising therapeutic approach for AR.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42413271	IL-3 beta from rat			Price

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