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Spectrophotometric Assay for Superoxide Dismutase Based on Tetrazolium Salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic Acid Hydrate Reduction by Xanthine-Xanthine Oxidase

Spectrophotometric Assay for Superoxide Dismutase Based on Tetrazolium Salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic Acid Hydrate Reduction by Xanthine-Xanthine Oxidase

H Ukeda, S Maeda, T Ishii, M Sawamura

Anal Biochem. 1997 Sep 5;251(2):206-9.

PMID: 9299017

Abstract:

XTT (3'-{1-[(phenylamino)-carbonyl]-3, 4-tetrazolium}bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate) was reduced to a water-soluble product with an absorbance maximum at about 470 nm by superoxide anion generated by xanthine-xanthine oxidase (XO). The rate of XTT reduction was linearly related to XO activity and the reduction was inhibited by superoxide dismutase (SOD). A perfect inhibition of the reduction of XTT by SOD was achieved, suggesting that XTT does not interact with XO. The present XTT-based assay had a higher sensitivity than a conventional nitroblue tetrazolium-based assay by a factor of 2.5 at pH 10.2. This method was applicable to the SOD assay in the pH range 8.0-10.2.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1072952501	3-methoxypyridine-4-boronic acid hydrate		1072952-50-1	Price

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