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Spectroscopic Investigation of Alloyed Quantum Dot-Based FRET to Cresyl Violet Dye

Spectroscopic Investigation of Alloyed Quantum Dot-Based FRET to Cresyl Violet Dye

M G Kotresh, K S Adarsh, M A Shivkumar, B G Mulimani, M I Savadatti, S R Inamdar

Luminescence. 2016 May;31(3):760-8.

PMID: 26333828

Abstract:

Quantum dots (QDs), bright luminescent semiconductor nanoparticles, have found numerous applications ranging from optoelectronics to bioimaging. Here, we present a systematic investigation of fluorescence resonance energy transfer (FRET) from hydrophilic ternary alloyed quantum dots (CdSeS/ZnS) to cresyl violet dye with a view to explore the effect of composition of QD donors on FRET efficiency. Fluorescence emission of QD is controlled by varying the composition of QD without altering the particle size. The results show that quantum yield of the QDs increases with increase in the emission wavelength. The FRET parameters such as spectral overlap J(λ), Förster distance R0, intermolecular distance (r), rate of energy transfer k(T)(r), and transfer efficiency (E) are determined by employing both steady-state and time-resolved fluorescence spectroscopy. Additionally, dynamic quenching is noticed to occur in the present FRET system. Stern-Volmer (K(D)) and bimolecular quenching constants (k(q)) are determined from the Stern-Volmer plot. It is observed that the transfer efficiency follows a linear dependence on the spectral overlap and the quantum yield of the donor as predicted by the Förster theory upon changing the composition of the QD.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS76901	CdSeS/ZnS alloyed quantum dots			Price

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