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Spiraeoside Inhibits Mast Cells Activation and IgE-mediated Allergic Responses by Suppressing Phospholipase C-γ-mediated Signaling

Spiraeoside Inhibits Mast Cells Activation and IgE-mediated Allergic Responses by Suppressing Phospholipase C-γ-mediated Signaling

Jung Kuk Kim, Young-Kyo Seo, Sehoon Park, Soo-Ah Park, Seyoung Lim, Susie Lee, Ohman Kwon, Jeong Kon Seo, Ung-Kyu Choi, Sung Ho Ryu, Pann-Ghill Suh

Biochem Cell Biol. 2015 Jun;93(3):227-35.

PMID: 25781488

Abstract:

Mast cells are responsible for IgE-mediated allergic responses through the secretion of various inflammatory cytokines and mediators. Therefore, the pharmacological regulation of mast cell activation is an important goal in the development of novel anti-allergic drugs. In this study, we found that spiraeoside (SP) inhibits mast cell activation and allergic responses in vivo. SP dose-dependently inhibited the degranulation induced by IgE-antigen (Ag) stimulation in RBL-2H3 mast cells without cytotoxic effects. At the molecular level, SP reduced the Ag-induced phosphorylation and subsequent activation of phospholipase C-γ2 (PLC-γ2). Moreover, SP inhibited the phosphorylation of spleen tyrosine kinase (Syk), linker for activation of T cells (LAT), and downstream MAPKs, such as ERK1/2, p38, and JNK, eventually attenuating expression of TNF-α and IL-4. Finally, we found that SP significantly inhibited IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. Taken together, our results strongly suggest that SP suppresses IgE-mediated mast cell activation and allergic responses by inhibiting Lyn-induced PLC-γ2/MAPK signaling in mast cells.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP20229565-A	Spiraeoside		20229-56-5	Price

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