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Spy&Go Purification of SpyTag-proteins Using pseudo-SpyCatcher to Access an Oligomerization Toolbox

Spy&Go Purification of SpyTag-proteins Using pseudo-SpyCatcher to Access an Oligomerization Toolbox

Irsyad N A Khairil Anuar, Anusuya Banerjee, Anthony H Keeble, Alberto Carella, Georgi I Nikov, Mark Howarth

Nat Commun. 2019 Apr 15;10(1):1734.

PMID: 30988307

Abstract:

Peptide tags are a key resource, introducing minimal change while enabling a consistent process to purify diverse proteins. However, peptide tags often provide minimal benefit post-purification. We previously designed SpyTag, forming an irreversible bond with its protein partner SpyCatcher. SpyTag provides an easy route to anchor, bridge or multimerize proteins. Here we establish Spy&Go, enabling protein purification using SpyTag. Through rational engineering we generated SpyDock, which captures SpyTag-fusions and allows efficient elution. Spy&Go enabled sensitive purification of SpyTag-fusions from Escherichia coli, giving superior purity than His-tag/nickel-nitrilotriacetic acid. Spy&Go allowed purification of mammalian-expressed, N-terminal, C-terminal or internal SpyTag. As an oligomerization toolbox, we established a panel of SpyCatcher-linked coiled coils, so SpyTag-fusions can be dimerized, trimerized, tetramerized, pentamerized, hexamerized or heptamerized. Assembling oligomers for Death Receptor 5 stimulation, we probed multivalency effects on cancer cell death. Spy&Go, combined with simple oligomerization, should have broad application for exploring multivalency in signaling.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP139139	Nitrilotriacetic acid		139-13-9	Price

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