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Stability and Activity of Lipase in Subcritical 1,1,1,2-tetrafluoroethane (R134a)

Stability and Activity of Lipase in Subcritical 1,1,1,2-tetrafluoroethane (R134a)

Gang Yu, Yong Xue, Wei Xu, Jing Zhang, Chang Hu Xue

J Ind Microbiol Biotechnol. 2007 Dec;34(12):793-8.

PMID: 17909872

Abstract:

The stability and activity of commercial immobilized lipase from Candida antarctica (Novozym 435) in subcritical 1,1,1,2-tetrafluoroethane (R134a) was investigated. The esterification of oleic acid with glycerol was studied as a model reaction in subcritical R134a and in solvent-free conditions. The results indicated that subcritical R134a treatment led to significant increase of activity of Novozym 435, and a maximum residual activity of 300% was measured at 4 MPa, 30 degrees C after 7 h incubation. No deactivation of Novozym 435 treated with subcritical R134a under different operation factors (pressure 2-8 MPa, temperature 30-60 degrees C, incubation time 1-12 h, water content 1:1, 1:2, 1:5 enzyme/water, depressurization rate 4 MPa/1 min, 4 MPa/30 min, 4 MPa/90 min) was observed. While the initial reaction rate was high in subcritical R134a, higher conversion was obtained in solvent-free conditions. Though the apparent conversion of the reaction is lower in subcritical R134a, it is more practicable, especially at low enzyme concentrations desired at commercial scales.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP811972	1,1,1,2-Tetrafluoroethane		811-97-2	Price

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