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Straightforward and Rapid Determination of Acrylamide in Coffee Beans by Means of HS-SPME/GC-MS

Straightforward and Rapid Determination of Acrylamide in Coffee Beans by Means of HS-SPME/GC-MS

Rafał Wawrzyniak, Beata Jasiewicz

Food Chem. 2019 Dec 15;301:125264.

PMID: 31377623

Abstract:

A straightforward and rapid preparation procedure for the extraction of the acrylamide from coffee beans, by means of the volatile silylated derivatives of acrylamide (AA) and headspace solid phase microextraction (HS-SPME) is described. Commercially available SPME fibre coated with polydimethylsiloxane (PDMS) was used. The silylation reactions of the AA were executed with N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA). The deuterium labelled d3-acrylamide was used as an internal standard. The acrylamide level was quantified using gas chromatography coupled with the mass spectrometry (GC-MS) in the selected ion monitoring (SIM) mode. The limit of quantification (LOQ) for measuring acrylamide was 3 µg/kg. The described method demonstrates satisfactory repeatability (RSD = 2.6%), with an intermediate precision (RSD = 9.4%) and recovery (99-105%).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP359867837	Polydimethylsiloxane-diacrylamide		359867-83-7	Price

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