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Streamlined Preparation of Immobilized Candida antarctica Lipase B

Karim Engelmark Cassimjee, Peter Hendil-Forssell, Alexey Volkov, Anne Krog, Jostein Malmo, Trond Erik V Aune, Wolfgang Knecht, Iain R Miskelly, Thomas S Moody, Maria Svedendahl Humble

ACS Omega. 2017 Dec 31;2(12):8674-8677.

PMID: 30023589

Abstract:

Candida antarctica lipase B (CalB) was efficiently expressed (6.2 g L-1) in Escherichia coli by utilizing an N-terminal tag cassette and the XylS/Pm expression system in a fed-batch bioreactor; subsequent direct binding to EziG from crude extracts resulted in an immobilized catalyst with superior activity to Novozym 435.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412270 Lipase immobilized from Candida antarctica Lipase immobilized from Candida antarctica Price
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