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Structural and Serological Characterization of the O82 Antigen of a Proteus Mirabilis Strain Isolated From a Patient in Poland

Structural and Serological Characterization of the O82 Antigen of a Proteus Mirabilis Strain Isolated From a Patient in Poland

Małgorzata Siwińska, Evgeniya A Levina, Alexander S Shashkov, Nadezhda A Kalinchuk, Dominika Drzewiecka, Yuriy A Knirel

Carbohydr Res. 2019 Dec 1;486:107831.

PMID: 31627049

Abstract:

P. mirabilis strains Kro 45 and Kwy 46 were isolated from the pus and the muscular fluid, respectively, of a hospitalized 61-year-old female in Łódź, Poland. Both strains demonstrated a good swarming ability on a solid medium, and the Dienes test for differentiation of swarming strains indicated their identity. The strains were serologically identical and did not belong to any of the known Proteus O1-O81 serogroups. In this work, we studied the O-specific polysaccharide (O antigen) of P. mirabilis Kwy46, which defines the immunospecificity of the strain. The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide, and the following structure of its oligosaccharide repeat (O-unit) was established by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy: where (S)-lac indicates an (S)-1-carboxyethyl group [an (S)-lactic acid residue], which forms an ether with a GlcNAc residue (so called glycolactilic acid). This structure is unique among Proteus O-polysaccharides but shares a trisaccharide fragment with that of P. mirabilis O5. Studies of the cross-reactivity between P. mirabilis Kwy 46 O antiserum/lipopolysaccharide and Proteus O1-O81 lipopolysaccharides/O antisera allowed identification of a putative Kwy 46 O-antigen epitope. Based on the data obtained, it is proposed to create a new O82 serogroup within the genus Proteus represented by the studied P. mirabilis isolates.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS7413156	Lipopolysaccharides from Proteus mirabilis			Price

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