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Structural Insight Into the Inhibition of Carbonic Anhydrase by the COX-2-selective Inhibitor Polmacoxib (CG100649)

Structural Insight Into the Inhibition of Carbonic Anhydrase by the COX-2-selective Inhibitor Polmacoxib (CG100649)

Hyun Tae Kim, Hyunju Cha, Kwang Yeon Hwang

Biochem Biophys Res Commun. 2016 Sep 9;478(1):1-6.

PMID: 27475498

Abstract:

Polmacoxib is not only a selective COX-2 inhibitor but also a potent inhibitor of carbonic anhydrases (CAs). Both CA I and CA II are highly expressed in the GI tract and kidneys, organs that are also thought to be the sites at which selective COX-2 inhibitors show their side effects. By inhibition assays, we show that both CA I and CA II are strongly inhibited by polmacoxib, while CA II also demonstrates direct competition with COX-2. To understand, at the molecular level, how polmacoxib interacts with CA I and II, we solved the first crystal structures of CA I and CA II in complex with polmacoxib, at 2.0 Å and 1.8 Å, respectively. Interestingly, three polmacoxib molecules bind to the active site of CA I, whereas only one molecule binds CA II. In the active site, the three molecules of polmacoxib organize itself along hydrophobic interaction as "stack-on-formation", and fully occupy a cone-shaped active pocket in CA I. The binding mode of polmacoxib to CA II was found different than its binding to celecoxib and valdecoxib. Our results provide structural insight into inhibition of CA I and CA II by polmacoxib, to assess its potential clinical efficacy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP181696333	COX-2 Inhibitor II - CAS 181696-33-3		181696-33-3	Price
AP416901581	COX-2 Inhibitor I - CAS 416901-58-1		416901-58-1	Price

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