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Structural Properties of Lipopolysaccharides From Rickettsia Typhi and Rickettsia Prowazekii and Their Chemical Similarity to the Lipopolysaccharide From Proteus Vulgaris OX19 Used in the Weil-Felix Test

Structural Properties of Lipopolysaccharides From Rickettsia Typhi and Rickettsia Prowazekii and Their Chemical Similarity to the Lipopolysaccharide From Proteus Vulgaris OX19 Used in the Weil-Felix Test

K I Amano, J C Williams, G A Dasch

Infect Immun. 1998 Mar;66(3):923-6.

PMID: 9488376

Abstract:

The lipopolysaccharides (LPSs) isolated from typhus group (TG) rickettsiae Rickettsia typhi and Rickettsia prowazekii were characterized by chemical analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by silver staining. LPSs from two species of TG rickettsiae contained glucose, 3-deoxy-D-manno-octulosonic acid, glucosamine, quinovosamine, phosphate, and fatty acids (beta-hydroxylmyristic acid and heneicosanoic acid) but not heptose. The O-polysaccharides of these LPSs were composed of glucose, glucosamine, quinovosamine, and phosphorylated hexosamine. Resolution of these LPSs by their apparent molecular masses by SDS-PAGE showed that they have a common ladder-like pattern. Based on the results of chemical composition and SDS-PAGE pattern, we suggest that these LPSs act as group-specific antigens. Furthermore, glucosamine, quinovosamine, and phosphorylated hexosamine were also found in the O-polysaccharide of the LPS from Proteus vulgaris OX19 used in the Weil-Felix test, suggesting that they may represent the antigens common to LPSs from TG rickettsiae and P. vulgaris OX19.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS7413157	Lipopolysaccharides from Proteus vulgaris			Price

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