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Structure and Expression of a Novel Member, FGF-16, on the Fibroblast Growth Factor Family

A Miyake, M Konishi, F H Martin, N A Hernday, K Ozaki, S Yamamoto, T Mikami, T Arakawa, N Itoh

Biochem Biophys Res Commun. 1998 Feb 4;243(1):148-52.

PMID: 9473496

Abstract:

We have isolated cDNA encoding a novel member (207 amino acids) of the FGF family from the rat heart by homology-based polymerase chain reaction. As this protein is the 16th documented member of the FGF family, we tentatively term it FGF-16. Among FGF family members, FGF-16 is most similar (73% amino acid identity) to FGF-9. We have also determined the structure of human FGF-16 with high amino acid sequence identity (98.6%) to rat FGF-16. Although the predicted FGF-16 amino acid sequence lacks a typical signal sequence, recombinant rat FGF-16 was efficiently secreted by Sf9 insect cells infected with recombinant baculovirus containing the cDNA. FGF-16 mRNA was predominantly expressed in the rat heart among the adult major tissues examined. The expression profile of FGF-16 mRNA was quite different from those of other members of the FGF family. In rat embryos, FGF-16 mRNA was predominantly expressed in the brown adipose tissue. However, the expression decreased greatly after birth. These results indicate that FGF-16 in embryos might play a role in development of the brown adipose tissue.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42413252 FGF-16 human FGF-16 human Price
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