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Structure and Properties of Phospholipid-Peptide Monolayers Containing Monomeric SP-B(1-25) I. Phases and Morphology by Epifluorescence Microscopy

Structure and Properties of Phospholipid-Peptide Monolayers Containing Monomeric SP-B(1-25) I. Phases and Morphology by Epifluorescence Microscopy

Nilanjana Biswas, Saratchandra Shanmukh, Alan J Waring, Frans Walther, Zhendong Wang, Y Chang, Robert H Notter, Richard A Dluhy

Biophys Chem. 2005 Mar 1;113(3):223-32.

PMID: 15620507

Abstract:

Epifluorescence microscopy was used to study the structure and phase behavior of phospholipid films containing a human-sequence monomeric SP-B(1-25) synthetic peptide (mSP-B(1-25)). Measurements were done directly at the air-water (A/W) interface on films in a Langmuir-Whilhelmy balance coupled to a fluorescence microscope and real-time detection system to yield an approximate optical resolution of 1 mum. Fluorescence was achieved by laser excitation of 2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero-3-PC (BODIPY-PC, concentration

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP67254288-A	1,2-Dioleoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt		67254-28-8	Price

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