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Structure and Transmembrane Topology of Slc11a1 TMD1-5 in Lipid Membranes

Structure and Transmembrane Topology of Slc11a1 TMD1-5 in Lipid Membranes

Jiantao Li, Lingling Wang, Li Wang, Fei Li

Biopolymers. 2012;98(3):224-33.

PMID: 22782564

Abstract:

The importance of solute carrier family 11 (Slc11) in divalent metal-ion transport has been well established. The core domains TMD1-5 and TMD6-10 of the proteins were modeled as a symmetric but inversely orientated arrangement with respect to membrane normal. In this article, the structures and transmembrane topologies of TMD1-5 of Slc11a1 incorporated with phospholipids 1-palmitoyl-2-oleoyl- sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)] (sodium salt) (POPG), and POPC/POPG (3:1) were explored using circular dichroism, fluorescence, and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopies. The segments TMD2-5 were inserted in lipid membranes mainly as an α-helix with orientations of helices along membrane normal. The tilt angles of the helices were in an order of TMD3 > TMD4 > TMD2 > TMD5 in these membranes. In contrast, TMD1 was partly inserted in membranes, leaving partial segment at membrane surface. The amount of the lipid component with negatively charged headgroups had an effect on both the helicity and orientation of the transmembrane domains (TMDs). Nevertheless, the helices maintained similar topologies in various membranes.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP1241726124	1,2-Dierucoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt	1241726-12-4	Price
AP200880428	1,2-Distearoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt	200880-42-8	Price
AP268550954	2-Oleoyl-1-palmitoyl-sn-glycero-3-phospho-rac-(1-glycerol) sodium salt	268550-95-4	Price

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