Home
Resources
Publications
Study of the Effects of Dispase in the Chemotherapy of Multicellular Tumour Spheroids of Small-Cell Lung Carcinoma in Man

Study of the Effects of Dispase in the Chemotherapy of Multicellular Tumour Spheroids of Small-Cell Lung Carcinoma in Man

H Matsuoka, M Okada, N Ishii, S Maeda

J Pharm Pharmacol. 1998 Mar;50(3):285-90.

PMID: 9600720

Abstract:

Multicellular tumour spheroids (MTS), diameter 650 microm, from PC-6, SBC-1 and NCL-H60 small-cell lung carcinoma cell-lines in man were prepared by the liquid overlay culture method and used to study the influence of treatment with dispase (bacterial neutral protease from Bacillus polymyxa, 1000 units mL(-1)) on the effectiveness of carboplatin, as determined by colony-forming assay. When carboplatin alone was used on monolayers the curve of survival fraction against concentration was exponential in shape, indicating that the drug was active against the monolayer. When MTS were treated with medium concentrations (10(-5) and 10(-4) M) of carboplatin alone the survival fraction-concentration curve showed that the effectiveness of the treatment was less than that against the monolayer. On treatment of MTS with carboplatin and dispase the survival fraction-concentration curve was similar to that obtained for the monolayer and the survival fraction of the core of the MTS was also less than when carboplatin alone was used. These results imply that dispase dissolves the intercellular matrix of the MTS enabling enhanced infiltration of carboplatin into the core of the MTS. Dispase thus indirectly increases the effectiveness of carboplatin.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP42613332-A	Dispase from Bacillus polymyxa		42613-33-2	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: