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Styrylpyrone Derivative (SPD) Induces Apoptosis in a caspase-7-dependent Manner in the Human Breast Cancer Cell Line MCF-7

Styrylpyrone Derivative (SPD) Induces Apoptosis in a caspase-7-dependent Manner in the Human Breast Cancer Cell Line MCF-7

Alvin Teck Chien Lee, Hawariah Lope Pihie Azimahtol, Ann Na Tan

Cancer Cell Int. 2003 Oct 4;3(1):16.

PMID: 14580263

Abstract:

BACKGROUND: Styrylpyrone derivative (SPD) is a plant-derived pharmacologically active compound extracted from Goniothalamus sp. Previously, we have reported that SPD inhibited the proliferation of MCF-7 human breast cancer cells by inducing apoptotic cell death, while having minimal effects on non-malignant cells. Here, we attempt to further elucidate the mode of action of SPD. RESULTS: We found that the intrinsic apoptotic pathway was invoked, with the accumulation of cytosolic cytochrome c and processing of the initiator caspase-9. Cleaved products of procaspase-8 were not detected. Next, the executioner caspase-7 was cleaved and activated in response to SPD treatment. To confirm that apoptosis was induced following caspase-7 activation, the caspase inhibitor Ac-DEVD-CHO was used. Pre-incubation of cells with this inhibitor reversed apoptosis levels and caspase-7 activity in SPD-treated cells to untreated levels. CONCLUSIONS: Taken together, these results suggest SPD as a potent antiproliferative agent on MCF-7 cells by inducing apoptosis in a caspase-7-dependent manner.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424994	Caspase 7 human			Price

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