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Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as Derivatization Reagents for the Determination of the Estrogens Estrone and 17alpha-ethinylestradiol by Gas Chromatography-Mass Spectrometry

Suitability of N,O-bis(trimethylsilyl)trifluoroacetamide and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide as Derivatization Reagents for the Determination of the Estrogens Estrone and 17alpha-ethinylestradiol by Gas Chromatography-Mass Spectrometry

Ali Shareef, Craig J Parnis, Michael J Angove, John D Wells, Bruce B Johnson

J Chromatogr A. 2004 Feb 13;1026(1-2):295-300.

PMID: 14763757

Abstract:

This paper describes apreviously unreported problem with the use of N,O-bis-(trimethylsilyl)trifluoroacetamide (BSTFA) and N-(tert-butyldimethylsilyl)-N-methyltrifluoroacetamide (MTBSTFA) to derivatise the natural hormone estrone (E1) and the synthetic estrogen 17alpha-ethinylestradiol (EE2). The resulting trimethylsilyl (TMS) and t-butyldimethylsilyl (TBS) derivatives of EE2 were partially converted to their respective El derivatives. Therefore, these reagents may not be suitable for simultaneous determination of estrogens in environmental samples, which raises questions about the reliability of results from some earlier studies.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP77377527-B	N-tert-Butyldimethylsilyl-N-methyltrifluoroacetamide		77377-52-7	Price

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