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Suitability of Ultra-High Performance Liquid Chromatography for the Determination of Fat-Soluble Nutritional Status (Vitamins A, E, D, and Individual Carotenoids)

Suitability of Ultra-High Performance Liquid Chromatography for the Determination of Fat-Soluble Nutritional Status (Vitamins A, E, D, and Individual Carotenoids)

F Granado-Lorencio, C Herrero-Barbudo, I Blanco-Navarro, B Pérez-Sacristán

Anal Bioanal Chem. 2010 Jun;397(3):1389-93.

PMID: 20358182

Abstract:

Our aim was to assess the suitability of ultra-high performance liquid chromatography (UHPLC) for the simultaneous determination of biomarkers of vitamins A (retinol, retinyl esters), E (alpha- and gamma-tocopherol), D (25-OH-vitamin D), and the major carotenoids in human serum to be used in clinical practice. UHPLC analysis was performed on HSS T3 column (2.1 x 100 mm; 1.8 microm) using gradient elution and UV-VIS detection. The system allows the simultaneous determination of retinol, retinyl palmitate, 25-OH-vitamin D, alpha- and gamma-tocopherol, lutein plus zeaxanthin, alpha-carotene, beta-carotene, alpha- and beta-cryptoxanthin and lycopene. The method showed a good linearity over the physiological range with an adequate accuracy in samples from quality control programs. Suitability of the method in clinical practice was tested by analyzing samples (n = 286) from patients. In conclusion, UHPLC constitutes a reliable approach for nutrient/biomarker profiling allowing the rapid, simultaneous and low-cost determination of vitamins A, E, and D (including vitamers and ester forms) and the major carotenoids in clinical practice.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
CS3104762	Beta Carotene System Suitability			Price

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