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Sulfonate Group-Modified FePtCu Nanoparticles as a Selective Probe for LDI-MS Analysis of Oligopeptides From a Peptide Mixture and Human Serum Proteins

Sulfonate Group-Modified FePtCu Nanoparticles as a Selective Probe for LDI-MS Analysis of Oligopeptides From a Peptide Mixture and Human Serum Proteins

Hideya Kawasaki, Tarui Akira, Takehiro Watanabe, Kazuyoshi Nozaki, Tetsu Yonezawa, Ryuichi Arakawa

Anal Bioanal Chem. 2009 Nov;395(5):1423-31.

PMID: 19760403

Abstract:

Bare FePtCu nanoparticles (NPs) are first prepared for laser desorption/ionization mass spectroscopy (LDI-MS) analysis as affinity probes to selectively trap oppositely charged analytes from a sample solution. Our present results demonstrate bare FePtCu NPs to be a potentially useful matrix for surface-assisted laser desorption/ionization mass spectroscopy (SALDI-MS), for the analysis of small proteins and peptides. The upper detectable mass range of peptides was approximately 5 kDa, and the detection limit for peptides approximately 5 fmol. Sulfonate group-modified FePtCu nanoparticles (FePtCu-SO(3)(-) NPs), with ionization being independent of the solution pH, can interact with a positively charged analyte, and the analyte-bound NPs can be separated from the reaction supernatant by centrifugation or an external magnetic field. An oligopeptide, Gly-Gly-Tyr-Arg (GGYR) from an oligopeptide mixture containing Asp-Asp-Asp-Asp (DDDD), Gly-Gly-Gly-Gly (GGGG) and GGYR, was detected using SALDI-MS with FePtCu-SO(3)(-) NPs employing electrostatic interaction. Furthermore, FePtCu-SO(3)(-) NPs can detect lysozyme (Lyz) in human serum through the electrostatic attraction between positively charged Lyz and FePtCu-SO(3)(-) NPs at pH 8, while detection of negatively charged albumin in human serum is not possible.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP70195209	Gly-Gly-Tyr-Arg		70195-20-9	Price

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