Home
Resources
Publications
Suppression of Thiol Exchange Reaction in the Determination of Reduced-Form Thiols by High-Performance Liquid Chromatography With Fluorescence Detection After Derivatization With Fluorogenic Benzofurazan Reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole

Suppression of Thiol Exchange Reaction in the Determination of Reduced-Form Thiols by High-Performance Liquid Chromatography With Fluorescence Detection After Derivatization With Fluorogenic Benzofurazan Reagent, 7-fluoro-2,1,3-benzoxadiazole-4-sulfonate and 4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole

Tomofumi Santa, Chiaki Aoyama, Takeshi Fukushima, Kazuhiro Imai, Takashi Funatsu

Biomed Chromatogr. Jun-Jul 2006;20(6-7):656-61.

PMID: 16779773

Abstract:

The derivatization of the reduced-form thiols with SBD-F (7-fluoro-2,1,3-benzoxadiazole-4-sulfonate) and ABD-F (4-aminosulfonyl-7-fluoro-2,1,3-benzoxadiazole) was studied. The yields of the derivatives of the reduced-form thiols (cysteine, homocysteine, reduced-form glutathione) with SBD-F at 60 degrees C for 45 min in the borate buffer (pH 9.3) were significantly decreased in the presence of the oxidized-form thiols (cystine, homocystine, oxidized-form glutathione) because of the thiol exchange reaction between the reduced-form and the oxidized-form thiols. The use of ABD-F at low temperature enabled the suppression of these thiol exchange reactions, and the recommended conditions were below 5 degrees C for 90 min in borate buffer (pH 9.3). These results suggest that ABD-F is a preferred derivatization reagent for the accurate determination of the reduced-form thiols in samples containing the oxidized-form thiols. In addition, it was also suggested that the derivatization of the reduced-form thiols should also be performed at low temperature when derivatization reagents such as o-phthalaldehyde (OPA) and monobromobimane (BrB) are used.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP142246488	4-Chloro-7-chlorosulfonyl-2,1,3-benzoxadiazole		142246-48-8	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: