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Surface-initiated Growth of Poly d(A-T) by Taq DNA Polymerase

Surface-initiated Growth of Poly d(A-T) by Taq DNA Polymerase

Young Shik Chi, Young Hwan Jung, Insung S Choi, Yang-Gyun Kim

Langmuir. 2005 May 10;21(10):4669-73.

PMID: 16032887

Abstract:

In this paper, we report surface-initiated d(A-T) polymerization by Taq DNA polymerase as a method for constructing DNA-tethered surfaces using an enzyme. The enzymatic polymerization was conducted successfully via two steps: tethering of oligo d(A-T)s onto the surface presenting carboxylic acids by amide coupling and surface-initiated polymerization using Taq DNA polymerase. In this enzymatic polymerization process, the design and construction of carboxylic acid-presenting surfaces were found to be an important factor: DNA growth did not occur on the gold surface coated only with the self-assembled monolayer (SAM) of 16-mercaptohexadecanoic acid (MHDA), but effectively proceeded on the surfaces presenting mixed SAMs of MHDA and 1-pentadecanethiol. The coupling of oligo d(A-T)s and the subsequent DNA polymerization reaction were characterized by polarized infrared external reflectance spectroscopy, ellipsometry, X-ray photoelectron spectroscopy, and atomic force microscopy.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP25276704	1-Pentadecanethiol		25276-70-4	Price

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