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Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of amyloid-β Peptide

Surface Plasmon Resonance Based Biosensors for Exploring the Influence of Alkaloids on Aggregation of amyloid-β Peptide

Bartłomiej Emil Kraziński, Jerzy Radecki, Hanna Radecka

Sensors (Basel). 2011;11(4):4030-42.

PMID: 22163834

Abstract:

The main objective of the presented study was the development of a simple analytical tool for exploring the influence of naturally occurring compounds on the aggregation of amyloid-β peptide (Aβ(40)) in order to find potential anti-neurodegenerative drugs. The gold discs used for surface plasmon resonance (SPR) measurements were modified with thioaliphatic acid. The surface functionalized with carboxylic groups was used for covalent attaching of Aβ(40) probe by creation of amide bonds in the presence of EDC/NHS. The modified SPR gold discs were used for exploring the Aβ(40) aggregation process in the presence of selected alkaloids: arecoline hydrobromide, pseudopelletierine hydrochloride, trigonelline hydrochloride and α-lobeline hydrochloride. The obtained results were discussed with other parameters which govern the phenomenon studied such as lipophilicity/hydrophilicy and Aβ(40)-alkaloid association constants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP134634	(−)-Lobeline hydrochloride		134-63-4	Price
AP6138416	Trigonelline hydrochloride		6138-41-6	Price

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