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Synthesis and Characterization of 4-iodophenylboronic Acid: A New Enhancer for the Horseradish Peroxidase-Catalyzed Chemiluminescent Oxidation of Luminol

L J Kricka, M Cooper, X Ji

Anal Biochem. 1996 Aug 15;240(1):119-25.

PMID: 8811887

Abstract:

4-Iodophenylboronic acid has been synthesized and shown to be a potent enhancer of the chemiluminescent horseradish peroxidase (Type VI-A)-catalyzed oxidation of luminol and isoluminol. The enhancer was effective (>100-fold enhancement) in the concentration range 10-1500 microM. Light emission in the presence of the enhancer peaked at 5-10 min after initiation of the reaction and then decayed very slowly. 4-Iodophenylboronic acid also enhanced reactions catalyzed by horseradish peroxidase Type IX. The detection limit for Type VI-A horseradish peroxidase was 509 amol, and optimum signal enhancement was obtained at 769 microM compared to 154 microM for 4-iodophenol under the same conditions.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP5122996 4-Iodophenylboronic acid 4-Iodophenylboronic acid 5122-99-6 Price
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