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Synthesis and Characterization of Stearyl Protamine and Investigation of Their Complexes With DNA for Gene Delivery

Synthesis and Characterization of Stearyl Protamine and Investigation of Their Complexes With DNA for Gene Delivery

Jiong Liu, Shengrong Guo, Zonghai Li, Li Liu, Jianren Gu

Colloids Surf B Biointerfaces. 2009 Oct 1;73(1):36-41.

PMID: 19481426

Abstract:

A novel gene carrier, stearyl protamine (SP) was synthesized by the reaction of natural protamine with an N-hydroxysuccinimide (NHS) ester of stearic acid. This SP was characterized by FT-IR, stearyl content measurement and dynamic light scattering (DLS). Then, the SP was evaluated as a gene delivery carrier. The SP with higher stearyl group content (SP3) can self-assemble into aggregates with an average diameter of ca. 110 nm in water and has relatively higher in vitro gene transfection efficiency than protamine and SP with lower stearyl group content. A possible mechanism is proposed for the formation of complex of SP/DNA. The SP also shows a low cytotoxicity similar to protamine.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP14464325	Stearic acid N-hydroxysuccinimide ester		14464-32-5	Price

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