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Synthesis and Secretion of a Nerve Growth-Stimulating Factor by Neonatal Mouse Astrocyte Cells in Vitro

Synthesis and Secretion of a Nerve Growth-Stimulating Factor by Neonatal Mouse Astrocyte Cells in Vitro

R H Tarris, M E Weichsel Jr, D A Fisher

Pediatr Res. 1986 Apr;20(4):367-72.

PMID: 3703628

Abstract:

Neonatal mouse astroglial cells cultured in a serum-free medium synthesize and secrete a trophic growth factor which resembles nerve growth factor (NGF). The NGF-like factor reacts with antiserum to beta subunit of NGF (beta-NGF) and, after labeling with [35S] cystine, migrates similarly to purified mouse beta-NGF in SDS polyacrylamide gel electrophoresis and Sephadex G-100 gel filtration systems. The astrocyte cell NGF-like factor displays beta-NGF-like neurite growth-promoting activity for the clonal rat pheochromocytoma (PC-12) cell line and this bioactivity is blocked by beta-NGF antiserum. These results indicate that NGF-like factor synthesized and secreted by astroglial cells, is similar, if not identical, to beta-NGF from the mouse submandibular gland and further support a potential role for NGF in the central nervous system.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR4248781	NGF-beta from mouse			Price

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