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Synthesis of 4-nitrophenyl Acetate Using Molecular Sieve-Immobilized Lipase From Bacillus Coagulans

Synthesis of 4-nitrophenyl Acetate Using Molecular Sieve-Immobilized Lipase From Bacillus Coagulans

Shilpa Raghuvanshi, Reena Gupta

J Ind Microbiol Biotechnol. 2009 Mar;36(3):401-7.

PMID: 19104860

Abstract:

Extracellular lipase from Bacillus coagulans BTS-3 was immobilized on (3 A x 1.5 mm) molecular sieve. The molecular sieve showed approximately 68.48% binding efficiency for lipase (specific activity 55 IU mg(-1)). The immobilized enzyme achieved approx 90% conversion of acetic acid and 4-nitrophenol (100 mM each) into 4-nitrophenyl acetate in n-heptane at 65 degrees C in 3 h. When alkane of C-chain length other than n-heptane was used as the organic solvent, the conversion of 4-nitrophenol and acetic acid was found to decrease. About 88.6% conversion of the reactants into ester was achieved when reactants were used at molar ratio of 1:1. The immobilized lipase brought about conversion of approximately 58% for esterification of 4-nitrophenol and acetic acid into 4-nitrophenyl acetate at a temperature of 65 degrees C after reuse for 5 cycles.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP830035	4-Nitrophenyl acetate		830-03-5	Price

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