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Synthesis of Calf Prochymosin (Prorennin) in Escherichia Coli

Synthesis of Calf Prochymosin (Prorennin) in Escherichia Coli

J S Emtage, S Angal, M T Doel, T J Harris, B Jenkins, G Lilley, P A Lowe

Proc Natl Acad Sci U S A. 1983 Jun;80(12):3671-5.

PMID: 6304731

Abstract:

A gene for calf prochymosin (prorennin) has been reconstructed from chemically synthesized oligodeoxyribonucleotides and cloned DNA copies of preprochymosin mRNA. This gene has been inserted into a bacterial expression plasmid containing the Escherichia coli tryptophan promoter and a bacterial ribosome binding site. Induction of transcription from the tryptophan promoter results in prochymosin synthesis at a level of up to 5% of total protein. The enzyme has been purified from bacteria by extraction with urea and chromatography on DEAE-cellulose and converted to enzymatically active chymosin by acidification and neutralization. Bacterially produced chymosin is as effective in clotting milk as the natural enzyme isolated from calf stomach.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9001983	Rennin from calf stomach		9001-98-3	Price

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