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Synthesis of Silica-Based Benzeneboronic Acid Affinity Materials and Application as Pre-Column in Coupled-Column High-Performance Liquid Chromatography

Synthesis of Silica-Based Benzeneboronic Acid Affinity Materials and Application as Pre-Column in Coupled-Column High-Performance Liquid Chromatography

Fanglou Li, Xinjie Zhao, Wenzhao Wang, Guowang Xu

Anal Chim Acta. 2006 Nov 24;580(2):181-7.

PMID: 17723771

Abstract:

Three silica-based benzeneboronic acid affinity materials were synthesized by using an m-aminobenzeneboronic acid as the ligand and using three different spacer arms. Under high-pressure, three affinity pre-columns were packed with these materials and the retention of every affinity pre-column with 11 urinary nucleosides was studied. With different spacer arms of boronic acid-substituted silica materials, the absorption to vicinal alcohols (cis-diols) and stability under acidic elution conditions are of great difference. Coupled-column liquid chromatographic methods for the direct analysis of urinary nucleosides were respectively established. As a result, two of three affinity pre-columns showed good chromatographic property in the on-line analysis of urinary nucleosides. The coupled-column system including pre-column I is the best with excellent linearity (R2>0.995), good recoveries (85.6-96.9%) and reproducibility (R.S.D.: 1.01-4.02%). The pre-column I could at least endure 150 repetitive injections of a 100 microL urinary sample.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793912	2-(e-cyanovinyl)benzeneboronic acid			Price
LS793929	(4-allylaminocarbonyl)benzeneboronic acid			Price

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