0

Synthesis of Symmetrical Thiol-Adenosine Conjugate and 5' thiol-RNA Preparation by Efficient One-Step Transcription

Faqing Huang, Yongliang Shi

Bioorg Med Chem Lett. 2010 Nov 1;20(21):6254-7.

PMID: 20832302

Abstract:

A symmetrical transcription initiator containing two adenosines and conjugated thiol functionality (ThioAMP dimer) is chemically synthesized. Transcription in the presence of ThioAMP dimer under the T7 Φ2.5 promoter yields 5' thiol-labeled RNA (5' HS-RNA) with up to 90% labeling efficiency, depending on the concentration ratio of ThioAMP dimer to ATP. The resulting 5' HS-RNA may be used directly or after thiopropyl Separose 6B affinity column purification. Biotinylation of 5' HS-RNA and formation of gold nanoparticle-RNA nanoplexes are demonstrated.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42415295 Caspase Inhibitor I, Biotin Conjugate Caspase Inhibitor I, Biotin Conjugate Price
qrcode