0

Synthetic Rabbit-Human Antibody Conjugate as a Control in Immunoassays for Immunoglobulin M Specific to Hepatitis E Virus

Kuo Zhang, Lunan Wang, Min Liu, Rui Zhang, Jinming Li

Virol J. 2010 May 20;7:101.

PMID: 20482892

Abstract:

Background:
In assays for anti-hepatitis E virus (HEV) immunoglobulin M (IgM), large volumes of the patient's sera cannot be easily obtained for use as a positive control. In this study, we investigated an alternative chemical method in which rabbit anti-HEV IgG was conjugated with human IgM and was used as a positive control in the anti-HEV IgM assay. Rabbit anti-HEV IgG was isolated from immune sera by chromatography on protein A-Sepharose and was conjugated with human IgM by using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) as a crosslinker.
Results:
The specific anti-HEV IgG antibody titer was 100,000 times that of the negative control, i.e., prebleed rabbit serum. The results of anti-HEV IgM enzyme-linked immunosobent assay showed that the antibody conjugate was similar to anti-HEV IgM antibodies produced in humans. The results of a stability experiment showed that the antibody conjugate was stable for use in external quality assessment or internal quality control trials.
Conclusions:
We concluded that the chemically conjugated rabbit-human antibody could be used instead of the traditional serum control as a positive control in the anti-HEV IgM assay.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412412 Anti-ACIN1 antibody produced in rabbit Anti-ACIN1 antibody produced in rabbit Price
IAR42413708 Anti-VEGFD antibody produced in rabbit Anti-VEGFD antibody produced in rabbit Price
IAR42413964 Anti-MYH3 antibody produced in rabbit Anti-MYH3 antibody produced in rabbit Price
IAR42414104 Anti-α-N-Catenin antibody produced in rabbit Anti-α-N-Catenin antibody produced in rabbit Price
IAR42417005 Anti-phospho-OSR1 (pThr185) antibody produced in rabbit Anti-phospho-OSR1 (pThr185) antibody produced in rabbit Price
IAR4245890 Anti-FOXC2 antibody produced in rabbit Anti-FOXC2 antibody produced in rabbit Price
qrcode