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TAIL-seq: Genome-Wide Determination of poly(A) Tail Length and 3' End Modifications

TAIL-seq: Genome-Wide Determination of poly(A) Tail Length and 3' End Modifications

Hyeshik Chang, Jaechul Lim, Minju Ha, V Narry Kim

Mol Cell. 2014 Mar 20;53(6):1044-52.

PMID: 24582499

Abstract:

Global investigation of the 3' extremity of mRNA (3'-terminome), despite its importance in gene regulation, has not been feasible due to technical challenges associated with homopolymeric sequences and relative paucity of mRNA. We here develop a method, TAIL-seq, to sequence the very end of mRNA molecules. TAIL-seq allows us to measure poly(A) tail length at the genomic scale. Median poly(A) length is 50-100 nt in HeLa and NIH 3T3 cells. Poly(A) length correlates with mRNA half-life, but not with translational efficiency. Surprisingly, we discover widespread uridylation and guanylation at the downstream of poly(A) tail. The U tails are generally attached to short poly(A) tails (<25 nt), while the G tails are found mainly on longer poly(A) tails (>40 nt), implicating their generic roles in mRNA stability control. TAIL-seq is a potent tool to dissect dynamic control of mRNA turnover and translational control, and to discover unforeseen features of RNA cleavage and tailing.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP26763199	poly(A)		26763-19-9	Price

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