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Temperature-Controlled Orientation of Proteins on Temperature-Responsive Grafted Polymer Brushes: Poly(butyl Methacrylate) vs Poly(butyl Acrylate): Morphology, Wetting, and Protein Adsorption

Temperature-Controlled Orientation of Proteins on Temperature-Responsive Grafted Polymer Brushes: Poly(butyl Methacrylate) vs Poly(butyl Acrylate): Morphology, Wetting, and Protein Adsorption

Kamil Awsiuk, Yurij Stetsyshyn, Joanna Raczkowska, Ostap Lishchynskyi, Paweł Dąbczyński, Andrij Kostruba, Halyna Ohar, Yana Shymborska, Svyatoslav Nastyshyn, Andrzej Budkowski

Biomacromolecules. 2019 Jun 10;20(6):2185-2197.

PMID: 31017770

Abstract:

Poly( n-butyl methacrylate) (PBMA) or poly( n-butyl acrylate) (PBA)-grafted brush coatings attached to glass were successfully prepared using atom-transfer radical polymerization "from the surface". The thicknesses and composition of the PBMA and PBA coatings were examined using ellipsometry and time-of-flight secondary ion mass spectrometry (ToF-SIMS), respectively. For PBMA, the glass-transition temperature constitutes a range close to the physiological limit, which is in contrast to PBA, where the glass-transition temperature is around -55 °C. Atomic force microscopy studies at different temperatures suggest a strong morphological transformation for PBMA coatings, in contrast to PBA, where such essential changes in the surface morphology are absent. Besides, for PBMA coatings, protein adsorption depicts a strong temperature dependence. The combination of bovine serum albumin and anti-IgG structure analysis with the principal component analysis of ToF-SIMS spectra revealed a different orientation of proteins adsorbed to PBMA coatings at different temperatures. In addition, the biological activity of anti-IgG molecules adsorbed at different temperatures was evaluated through tracing the specific binding with goat IgG.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9003638-A	Poly(butyl methacrylate), MW 800,000		9003-63-8	Price

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