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Temperature-jump Studies on Benzeneboronic Acid-Serine Protease Interactions. Subtilisin and Alpha-Chymotrypsin

H Nakatani, K Hanai, Y Uehara, K Hiromi

J Biochem. 1975 Apr;77(4):905-8.

PMID: 238970

Abstract:

The interactions of benzeneboronic acid (BBA) as a transition state analog with subtilisin (EC 3.4.21.4) and with alpha-chymotrypsin (EC 3.4.21.1) were investigated kinetically by the temperature-jump method using pH indicators. For both enzymes, the concentration dependence of the relaxation time was consistent with a two-step mechanism involving a fast bimolecular association followed by a slow, unimolecular process. The possibility of a trigonal-tetrahedral interconversion of BBA at the active site of the enzyme is discussed.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
LS793922 4-(N-cyclohexylsulphonamido)benzeneboronic acid 4-(N-cyclohexylsulphonamido)benzeneboronic acid Price
LS793929 (4-allylaminocarbonyl)benzeneboronic acid (4-allylaminocarbonyl)benzeneboronic acid Price
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