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The Application of 8-aminoguanosine Triphosphate, a New Inhibitor of GTP Cyclohydrolase I, to the Purification of the Enzyme From Human Liver

The Application of 8-aminoguanosine Triphosphate, a New Inhibitor of GTP Cyclohydrolase I, to the Purification of the Enzyme From Human Liver

N Blau, A Niederwieser

Biochim Biophys Acta. 1986 Jan 15;880(1):26-31.

PMID: 3753653

Abstract:

GTP cyclohydrolase I from human liver and Escherichia coli is competitively inhibited by 8-aminoguanosine triphosphate with a dissociation constant (Ki) of 0.25 mumol/l. 8-Aminoguanosine triphosphate, prepared from GTP and hydroxylamine-O-sulfonic acid, was coupled to Sepharose 4B and used as an affinity adsorbent for a 309-fold purification of GTP cyclohydrolase I from human liver. GTP cyclohydrolase I from human liver is a relatively heat-stable enzyme with a half-life of 2 min at 80 degrees C, an isoelectric point (pI) of about 5.6, and a Km for GTP of 31 mumol/l. Addition of KCl (0.3 mol/l) increased the Km to 153 mumol/l. No cofactors were required for activity. L-erythro-5,6,7,8-Tetrahydrobiopterin, L-erythro-7,8-dihydrobiopterin, L-sepiapterin and 8-aminoguanosine triphosphate were strong inhibitors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP3868324	8-Aminoguanosine		3868-32-4	Price

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